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. 2018 Jan 24;19(3):e44871. doi: 10.15252/embr.201744871

Figure 3. Che‐1 regulates transcription and strongly cooperates with the transcriptional machinery on chromatin of B‐ALL cells.

Figure 3

  1. Intersection of spike‐in‐normalized differential expressed genes in LAL‐B and NALM‐6 cell lines. The 842 genes downregulated in both cell lines show a strong enrichment in several functional clusters.
  2. Venn diagram of Che‐1 peak sets in LAL‐B and NALM‐6 cell lines computed by bedtools intersect.
  3. ChIP‐seq signal, centered on 2,205 TSS sites extracted from the 842 RNA‐seq downregulated signature. The Deeptools Heatmap plot shows an enrichment of Che‐1 position with respect to both inputs.
  4. Genome Browser Screenshots of ChIP‐seq bigWig signal on cell‐cycle‐related genes promoters.
  5. Quantitative ChIP analysis (ChIP–qPCR) of Che‐1 enrichment on CDK13, PLK1, and PTEN promoters in NALM‐6 and LAL‐B cells. H2AB (Histone Cluster 2 H2A Family Member B) was used as negative control. Data are expressed as percent of input. Error bars represent the standard error of three different experiments. **P ≤ 0.01; ***P ≤ 0.001 by Student's t‐test.