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LCL were transfected with specific siRNA and then treated with 0, 0.01, 0.1, 1, 10, 25, 50, 100, and 500 μM TCN for 72 h. Cell survival was determined using CYQUANT assay. Knockdown efficiency was determined using qRT–PCR. Each point shows the mean values for three independent experiments; error bars represent ± SEM.
