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. 2018 Jan 22;15(4):4255–4261. doi: 10.3892/ol.2018.7840

Figure 5.

Figure 5.

Blood-filled caverns unlined by endothelial cells are generated from blood vessel dilation followed by endothelial detachment as demonstrated by Sirius red staining of INS-1 tumors. (A-C) Represent different sites in the same slide. (A) Certain endothelial cells were not connected (arrows) with adjacent tumor cells in dilated blood vessels or blood-filled caverns (marked as a). (B) Detached endothelial cells formed a cluster (arrows) in blood-filled caverns unlined by endothelial cells (marked as b). (C) In specific blood-filled caverns (marked as c), collagen deposition was observed in a particular site of the lakeside (arrowheads). Endothelial cell detachment (arrow) was also observed. Scale bar, 50 µm.