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. 2018 Jan 26;15(4):4535–4540. doi: 10.3892/ol.2018.7878

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Figure 4. — HNF1A-AS1 promotes BC cell migration and invasion in vitro. (A) Representative images of cell migration experiment for T24 and 5637 BC cells. The number of migrated cells decreased visually when cells were depleted using HNF1A-AS1. (B) Migrated cells on the lower surface of the chamber were stained with 1% crystal violet and counted. The knockdown of HNF1A-AS1 in T24 and 5637 cells decreased the number of migrated cells. *P<0.05 vs. control. (C) Representative images of cell invasion assay for BC cell lines following transfection with the specific shRNA against HNF1A-AS1. (D) Quantification of invaded cells on the lower surface of the chamber for the invasion assays. The knockdown of HNF1A-AS1 decreased the cell invasion to ~50% compared with that in the control group. *P<0.05 vs. control. HNF1A-AS1, hepatocyte nuclear factor 1A-antisense RNA1; BC, bladder cancer; sh, short hairpin RNA; NC, negative control.