Figure 1.

Simplified pedigree of the analyzed family. (A) Symbols with black upper corner indicate individuals affected by Parkinson’s disease (PD). Age of onset of PD (years) is reported; in the patients’ relatives, age at last examination or age at death is reported. The result of leucine-rich repeat kinase 2 (LRRK2) genetic screening for the mutation was either wild type (WT) or heterozygous carrier (E193K). Gender of healthy siblings has been masked to protect the anonymity of the families. (B) The table summarizes clinical data of the three E193K carriers. (C) Sequencing of PCR product from exon 6 of WT and mutant alleles. The upper chromatogram of the portion of the sequencing gel shows the wild allele and the lower the heterozygous mutant allele.