Skip to main content
. 2018 Feb 28;11:64. doi: 10.3389/fnmol.2018.00064

Figure 5.

Figure 5

E193K mitochondria are more sensitive to MPP+. (A) Primary fibroblasts obtained from healthy individuals, E193K or G2019S carriers were treated with 1 or 3 mM MPP+ for 24 h and cell vitality was measured by the means of MTT assays. The graph reports relative viability. Data are shown as mean ± SE, n = 6; ***p < 0.001 vs. control conditions, same genotype, #p < 0.05 vs. WT, same treatment. (B) Oxygen consumption assessed by high resolution respirometry of primary fibroblasts after 24 h treatment with 1 mM MPP+. The graph reports routine activity (routine respiration), complex I and complex II—dependent activity (upon treatment with malate, glutamate and succinate) and maximal activity (maximal uncoupled respiration after FCCP injection). Data show means ± SE, n = 4; *, **p < 0.05, 0.01 vs. control conditions, same genotype. (C) Primary fibroblasts obtained from healthy individuals, G2019S or E193K carriers were treated with 1 mM MPP+ for 24 h and then incubated with Mitosox to measure the reactive oxygen species (ROS) production. Scale bar = 25 μM. (D) The graph reports the intensity of the Mitosox signal, expressed as integrated density. Data are expressed as mean ± SE, n = 4 (20 cells/experiment); #p < 0.05 vs. control condition, same genotype. *, **p < 0.05, 0.01 vs. WT, same treatment.