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. 2018 Jan 22;15(4):4365–4375. doi: 10.3892/ol.2018.7837

Table IV.

Dynamic detection of tumor tissue DNA and serial plasma circulating tumor DNA driver mutations in 11 patients with colorectal cancer.

Case Gene Chromosome Transcript Exon Nucleotide variation Amino acid variation Mutation frequency of tumor tissue (%) Cosmid Mutation frequency of preoperative plasma (%) ALT-Depth Depth Mutation frequency of 1 month postoperative plasma (%) ALT-Depth Depth Mutation frequency of 3 month postoperative plasma(%) ALT-Depth Depth Mutation frequency of 5 mo postoperative plasma(%) ALT-Depth Depth
H1001 ERBB2 chr17 NM_004448 exon21 c.G2524A p.V842I 34.81 COSM14065 1.26 35 2,787 0 0 0 – – – 0 0 0
H1001 APC chr5 NM_000038 exon6 c.G568T p.E190X 32.16 COSM258874 0.87 23 2,653 0 0 0 – – – 0 0 0
H1001 TP53 chr17 NM_000546 exon5 c.G524A p.R175H 31.4 COSM10648 1.02 16 1,575 0.46 5 1,081 – – – 0 0 0
H1001 ERBB4 chr2 NM_005235 exon28 c.T3492A p.N1164K 26.48 1.03 33 3,215 0 0 0 – – – 0 0 0
H1001 KRAS chr12 NM_004985 exon2 c.G35A p.G12D 25.66 COSM521 0.44 10 2,254 0 0 0 – – – 0 0 0
H1002 TP53 chr17 NM_000546 exon8 c.G818A p.R273H 81.35 COSM10660 0 0 0 0 0 0 0.13 3 2,352 0 0 0
H1002 APC chr5 NM_000038 exon16 c.G4222T p.E1408X 80.49 COSM18822 0 0 0 0 0 0 0 0 0 0 0 0
H1002 APC chr5 NM_000038 exon16 c.T2735A p.L912X 78.89 COSM4167198 0 0 0 0.26 2 768 0 0 0 0 0 0
H1003 TP53 chr17 NM_000546 exon8 c.A857G p.E286G 61.08 COSM43565 8.95 233 2,602 11.32 124 1,100 42.39 993 2,342 71.4 1,990 2,787
H1003 MLH3 chr14 NM_014381 exon2 c.G1163A p.S388N 7.23 0.11 3 2,790 0 0 0 0 0 0 0 0 0
H1003 KMT2D chr12 NM_003482 exon31 c.G7489A p.A2497T 5.17 0 0 0 0 0 0 0 0 0 0 0 0
H1003 PIK3CA chr3 NM_006218 exon9 c.G1624A p.E542K 0 COSM760 0 0 0 0 0 0 12.66 195 1,542 19.96 505 2,532
H1003 APC chr5 NM_000038 exon6 c.617delC p.T206fs 68.83 8.58 159 1,854 15.69 96 612 0 0 0 76.53 2,045 2,672
H1003 PTEN chr10 NM_001304717 exon1 c.19_21del p.7_7del 5.76 0 0 0 0 0 0 0 0 0 0 0 0
H1004 CTNNB1 chr3 NM_001904 exon3 c.A121G p.T41A 65.85 COSM5664 0.27 9 3,292 0 0 0 0 0 0 0 0 0
H1004 BRCA2 chr13 NM_000059 exon10 c.A832G p.S278G 33.18 0.22 6 2,735 0.53 6 1,125 0 0 0 0 0 0
H1004 PIK3CA chr3 NM_006218 exon2 c.A331G p.K111E 31.66 COSM13570 0.81 25 3,076 0 0 0 0 0 0 0 0 0
H1004 KRAS chr12 NM_004985 exon2 c.G38A p.G13D 30.32 COSM532 1 19 1,897 0 0 0 0 0 0 0 0 0
H1004 BRCA2 chr13 NM_000059 exon13 c.C7006T p.R2336C 30.17 COSM432312 0.56 15 2,702 0 0 0 0 0 0 0 0 0
H1004 FZR1 chr19 NM_016263 exon7 c.C542T p.A181V 28.37 0.33 9 2,733 0.21 2 937 0 0 0 0 0 0
H1004 SLIT1 chr10 NM_003061 exon11 c.G1054A p.A352T 27.5 0 0 0 0 0 0 0.27 2 740 0 0 0
H1004 CYP19A1 chr15 NM_000103 exon10 c.C1285T p.P429S 27.34 0.41 10 2,458 0.34 4 1,178 0.52 6 1,163 0 0 0
H1004 KMT2D chr12 NM_003482 exon11 c.C3868T p.R1290W 22.82 COSM4042398 0.59 12 2,031 0 0 0 0.22 2 913 0 0 0
H1004 KMT2B chr19 NM_014727 exon28 c.T6737G p.V2246G 7.95 0 0 0 0 0 0 0 0 0 0 0 0
H1004 KMT2B chr19 NM_014727 exon28 c.T6746G p.V2249G 5.39 0.51 3 587 0 0 0 0 0 0 0 0 0
H1004 ARID1A chr1 NM_006015 exon1 c.433delC p.P145fs 25.28 0 0 0 0 0 0 0 0 0 0 0 0
H1004 AKAP9 chr7 NM_005751 exon19 c.5010_5013del p.G1670fs 22.1 0 0 0 0 0 0 0 0 0 0 0 0
H1005 EPCAM chr2 NM_002354 exon1 c.T25G p.F9V 6.32 0 0 0 0 0 0 0 0 0 – – –
H1006 RB1 chr13 NM_000321 exon1 c.A13C p.T5P 10.03 0 0 0 0 0 0 0 0 0 0 0 0
H1006 KMT2B chr19 NM_014727 exon28 c.T6746G p.V2249G 5.22 0 0 0 0 0 0 0 0 0 0 0 0
H1006 ARID1A chr1 NM_006015 exon1 c.A653C p.N218T 5.07 0 0 0 0 0 0 0 0 0 0 0 0
H1006 APC chr5 NM_000038 exon16 c.4217_4218del p.Q1406fs 49.86 COSM5007948 0 0 0 0 0 0 0 0 0 0 0 0
H1006 TP53 chr17 NM_000546 exon7 c.749_756del p.P250fs 36.1 0 0 0 0 0 0 0 0 0 0 0 0
H1007 SMAD4 chr18 NM_005359 exon9 c.G988C p.E330Q 41.96 COSM14240 0 0 0 0 0 0 0 0 0 0 0 0
H1007 TP53 chr17 NM_000546 exon5 c.G473A p.R158H 40.38 COSM10690 0.21 2 972 0 0 0 0 0 0 0 0 0
H1007 PIK3CA chr3 NM_006218 exon5 c.A1034T p.N345I 34.47 COSM94978 0 0 0 0 0 0 0 0 0 0.48 5 1,058
H1007 FBXW7 chr4 NM_018315 exon8 c.C1018T p.H340Y 32.41 COSM28798 0.28 3 1,086 0 0 0 0 0 0 0 0 0
H1007 KRAS chr12 NM_004985 exon2 c.G35T p.G12V 26.5 COSM520 0 0 0 0 0 0 0 0 0 0 0 0
H1007 CDH1 chr16 NM_004360 exon9 c.G1259A p.G420D 18.71 0 0 0 0 0 0 0 0 0 0 0 0
H1007 ATM chr11 NM_000051 exon17 c.T2570G p.L857R 14.45 0 0 0 0 0 0 0 0 0 0 0 0
H1007 SMAD2 chr18 NM_005901 exon9 c.1009_1024del p.R337fs 29.56 0 0 0 0 0 0 0 0 0 0 0 0
H1008 KRAS chr12 NM_004985 exon3 c.A183T p.Q61H 79.42 COSM555 3.26 52 1,593 0 0 0 0 0 0 0 0 0
H1008 SMAD4 chr18 NM_005359 exon12 c.T1619G p.L540R 68.95 COSM14165 0 0 0 0 0 0 0 0 0 0 0 0
H1008 UGT1A1 chr2 NM_000463 exon1 c.G323A p.R108H 36.8 COSM1018132 0.46 7 1,508 0.2 4 1,992 0.17 3 1,756 0 0 0
H1008 ATIC chr2 NM_004044 exon6 c.G515A p.R172H 18.27 COSM1183927 0 0 0 0 0 0 0 0 0 0 0 0
H1009 KRAS chr12 NM_004985 exon2 c.G35A p.G12D 33.98 COSM521 0.54 3 553 0 0 0 0 0 0 0 0 0
H1009 RB1 chr13 NM_000321 exon1 c.A13C p.T5P 7.94 0 0 0 0 0 0 0 0 0 0 0 0
H1009 ARID1A chr1 NM_006015 exon1 c.A317C p.N106T 6.77 0 0 0 0 0 0 0 0 0 0 0 0
H1009 MSH6 chr2 NM_000179 exon1 c.A256C p.T86P 5.56 0 0 0 0 0 0 0 0 0 0 0 0
H1009 ARID1A chr1 NM_006015 exon20 c.5125_5131del p.L1709fs 21.35 0 0 0 0 0 0 0 0 0 0 0 0
H1010 KMT2B chr19 NM_014727 exon1 c.T266G p.V89G 7.46 0 0 0 0 0 0 0 0 0 0 0 0
H1010 KMT2B chr19 NM_014727 exon28 c.T6746G p.V2249G 6.78 0 0 0 0 0 0 0 0 0 0 0 0
H1010 KMT2D chr12 NM_003482 exon11 c.T2987G p.V996G 5.41 0 0 0 0 0 0 0 0 0 0 0 0
H1010 TP53 chr17 NM_000546 exon7 c.715_720del p.239_240del 11.72 COSM45055 0 0 0 0 0 0 0.19 3 1,624 0 0 0
H1010 APC chr5 NM_000038 exon16 c.2025delA p.T675fs 7.35 0 0 0 0 0 0 0 0 0 0 0 0
H1011 TP53 chr17 NM_000546 exon8 c.C844T p.R282W 43.17 COSM10704 0.39 6 1,533 0 0 0 0 0 0 0 0 0
H1011 PIK3CA chr3 NM_006218 exon5 c.T1035A p.N345K 40.51 COSM754 0 0 0 0 0 0 0 0 0 0 0 0
H1011 RB1 chr13 NM_000321 exon1 c.A13C p.T5P 8.73 9.42 13 138 0 0 0 0 0 0 0 0 0
H1011 TP53 chr17 NM_000546 exon5 c.G404A p.C135Y 8.28 COSM10801 0.19 3 1,548 0 0 0 0 0 0 0 0 0
H1011 KMT2B chr19 NM_014727 exon1 c.T248G p.L83R 7.96 0 0 0 0 0 0 0 0 0 0 0 0
H1011 KMT2B chr19 NM_014727 exon28 c.T6746G p.V2249G 7.06 0 0 0 0 0 0 0 0 0 0 0 0
H1011 ERBB2 chr17 NM_004448 exon1 c.T25G p.W9G 6.37 0 0 0 0 0 0 0 0 0 0 0 0
H1011 KMT2B chr19 NM_014727 exon28 c.T6737G p.V2246G 5.67 0 0 0 0 0 0 0 0 0 0 0 0
H1011 CREBBP chr16 NM_004380 exon30 c.A4931C p.N1644T 5.61 0 0 0 0 0 0 0 0 0 0 0 0
H1011 APC chr5 NM_000038 exon16 c.G4057T p.E1353X 5.57 COSM19048 0 0 0 0 0 0 0 0 0 0 0 0
H1011 PTCH1 chr9 NM_000264 exon1 c.A26C p.E9A 5.41 0 0 0 0 0 0 0 0 0 0 0 0
H1011 PTPRK chr6 NM_002844 exon15 c.C2432T p.T811I 5.39 0 0 0 0 0 0 0 0 0 0 0 0
H1011 TYMP chr22 NM_001953 exon8 c.T941G p.L314R 5.31 0 0 0 0 0 0 0 0 0 0 0 0
H1011 RB1 chr13 NM_000321 exon1 c.A24C p.K8N 5.29 0 0 0 0 0 0 0 0 0 0 0 0
H1011 TYMP chr22 NM_001953 exon9 c.T1175G p.V392G 5.22 0 0 0 0 0 0 0 0 0 0 0 0

ALT, alteration.