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. 2018 Feb 28;9:223. doi: 10.3389/fpls.2018.00223

FIGURE 3.

FIGURE 3

Heterologous expression of OsZOUs in the Atzou-4 mutant. (A–D) Seed phenotypes of Col-0, Atzou-4, OsZOU-1, and OsZOU-2 transgenic lines (the seed phenotypes of independent transgenic events OsZOU-1#1, #4, #5 and OsZOU-2#1, #3, #5 were observed, but only images from OsZOU-1#4 and OsZOU-2#3, considered representative for all other events, are presented here). The seeds from Col-0 are full (A) while those from zou-4 are abnormally shriveled (B). In OsZOU-1 transgenic lines, the seeds are plump and some show irregular shapes, indicated by black arrow heads (C), while the seeds of OsZOU-2 transgenic lines were shriveled, similar to the Atzou-4 mutant (D). Scale bars = 1 mm. (E) qPCR analyses were performed to analyze the expression levels of OsZOUs in transgenic seeds at the heart stage. Five independent transgenic lines from each were examined and the un-transformed Atzou-4 mutants were selected as a negative control. The expression of OsZOUs was quantified relative to AtZOU expression in Col-0 seeds. Error bars represent the standard deviation calculated from three biological replicates, each containing three consecutive siliques at the mid to late heart stage, with AtEIF4 used as the reference gene. Student’s t-test value: ∗∗p < 0.01.