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. 2018 Feb 2;8(5):1243–1255. doi: 10.7150/thno.22856

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Examination of the restoration and localization of dysferlin protein in EXOdysf-treated murine dysferlin-deficient (DYSF^-/-) myotubes. The restoration of dysferlin protein was examined 48 h after the addition of 100 μg/mL EXOdysf in DYSF^-/- myotubes. (A) Western blot to measure the level of dysferlin protein in EXOdysf-treated DYSF^-/- myotubes. GAPDH was used as a loading control and 20 μgor 200 μg total protein from cell lysates or exosomes was loaded, respectively. (B) Confocal fluorescence microscopy images to reflect the localization of restored dysferlin protein in EXOdysf-treated DYSF^-/- myotubes. Nuclei were counterstained with DAPI (blue) (scale bar = 30μm; for boxed areas, scale bar= 15 μm). Arrowheads refer to the co-localization of caveolin-3 (red) and exosome (green).