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. 2018 Feb 2;8(5):1376–1388. doi: 10.7150/thno.22717

Figure 3.

Figure 3

Glucose uptake and consumption are increased in CAFs. A) The cellular 18F-FDG concentration and the media glucose consumption were increased in both PDGF-induced and HCT116-CM-induced CAFs. Fibroblasts were cultured in glucose-free medium with 18F-FDG at a concentration of 1 μCi/mL. B) The cellular 18F-FDG concentration and the media glucose consumption were increased in isolated CAFs C) The cellular 18F-FDG concentration and the media glucose consumption were increased in the mixed populations of colon cancer HCT116 cells with NAFs or induced CAFs. HCT116 cells and fibroblasts for each injection were 3 × 105 and 1.5 × 106. D) The CAFs were more sensitive to a glycolysis inhibitor (3-brodomopyruvate, 3-BrPA) than the NAFs were. The cells were treated for 24 h. E) Representative cell images after 3-BrPA treatment. The fibroblasts were treated with 3-BrPA (50 μM) for 8 h. F) Representative cell images of colon cancer HCT116 cells co-cultured with NAFs or induced CAFs treated with 3-BrPA (50 μM) for 8 h. Red arrows point to fibroblasts, blue arrows point to cancer cells. **: p < 0.01, ***: p < 0.001.