Figure 3.

MPs and Exos exert immunosuppressive functions on T lymphocyte subsets. (A) Proliferation of Concanavalin A-activated murine splenocytes cultured alone for 3 days (Ctrl) or incubated with increasing amounts (ng) of Exos (left) or MPs (right) from naïve or IFN-γ primed MSCs (n=5 biological replicates). (B) Percentage of CD8⁺IFN-γ⁺ cytotoxic T cells, CD4⁺IFN-γ⁺ Th1 cells, CD4⁺IL-10⁺ Tr1 cells and CD4⁺CD25⁺Foxp3⁺ Treg cells in splenocytes (n=4) when incubated alone or with naïve MSCs and 25 ng of Exos or MPs. (C) Proliferation of Concanavalin A-activated sorted CD4⁺ (left) and CD8⁺ (right) T lymphocytes cultured alone for 3 days (Ctrl) or incubated with naïve MSCs and 50 ng of Exos or MPs from naïve or IFN-γ primed MSCs (n=3 biological replicates). Statistical analysis used a non-parametric Kruskal-Wallis test with Dunn's multiple comparison post-test. *: p<0.05 compared to Ctrl.