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. 2018 Feb 3;8(5):1411–1420. doi: 10.7150/thno.22252

Figure 3.

Figure 3

Fluorescence imaging in cells. HeLa cells were pre-incubated with the following agents: (a) no treatment; (b) 1 mM NMM for 30 min; (c) 1 mM NMM for 30 min and 300 μM Cys for 30 min; (d) 1 mM NMM for 30 min and 300 µM Hcy for 30 min; (e) 1 mM NMM for 30 min and 1 mM GSH-MEE for 60 min. Cells were then washed with Dulbecco's phosphate-buffered saline (DPBS) and incubated with 10 µM MNDA for 30 min. After washing with DPBS, fluorescence images were acquired by confocal microscopy. Blue: ex. 405 nm/em. 430-455 nm, green: ex. 473 nm/em. 490-540 nm. Scale bar: 10 µm.