Figure 2.

Abnormal miR-30d reduces the expression levels of MMP-2 and MMP-9, and induces morphological changes in SK-ES-1 cells. (A) Blots and (B) quantification of the western blot assay revealed that the expression levels of MMP-2 and MMP-9 were significantly decreased in the miR-30d mimic group compared with those in the untreated group (**P<0.01). (C) Staining with the fluorescent DNA-binding dye Hoechst 33258 revealed that SK-ES-1 cells transfected with the miR30d mimic possessed condensed and fragmented nuclei, which are typical morphological features of apoptotic cells. By contrast, no morphological signs of apoptosis were observed in cells of the untreated group or scrambled-sequence mimic group (magnification, ×200). miR, microRNA; MMP, matrix metalloproteinase.