Figure 3.

Overexpression of miR-30d arrests the cell cycle at the S phase and leads to apoptosis of SK-ES-1 cells. (A) Flow cytometric assay showed that abnormal miR-30d led to the accumulation of S-phase SK-ES-1 cells and the (B) statistical analysis revealed a significant difference in S-phase cell number between the miR-30d mimic group and the untreated group (***P<0.001). (C) Flow cytometric assay and (D) statistical analysis showed that the proportions of early and late apoptotic cells were significantly elevated in the miR-30d mimic group, compared with those in the untreated group (**P<0.01). miR, microRNA.