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. 2018 Feb 1;8(2):20. doi: 10.3390/bs8020020

Figure 2.

Figure 2

Effect of pedunculopontine nucleus (PPN) neurotoxic lesion on the mRNA expression of proteins related to dopaminergic homeostasis in nigral and striatal tissue. The study was by semi-quantitative RT-PCR, expressed as percentage of b-actin 48 h and 7 days after PPN lesion. Note that for the three proteins studied there were no significant differences between experimental groups 48 h after PPN injury (p > 0.05). (A) Comparison between experimental groups of tyrosine hydroxilase mRNA expression in nigral tissue 7 days after PPN lesion (F(2, 12) = 5.34 p < 0.05); (B) Comparison between experimental groups of vesicular monoamine transporter 2 (VMAT2) mRNA expression in nigral tissue 7 days after PPN lesion (F(2, 12) = 6.16 p < 0.01); (C) Comparison between experimental groups of dopamine transporter (DAT) mRNA expression in striatal tissue 7 days after PPN lesion (F(2, 12) = 20.29 p < 0.001); (D) Agarose gel electrophoresis bands representative of the semiquantitative RT-PCR study for each of the markers studied. Experimental groups: non-operated (n = 5), PPN lesion (n = 5), and sham-operated rats (n = 5). * p < 0.05; ** p < 0.01; *** p < 0.001. Bars represent mean ± SEM.