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. 2018 Feb 11;7(2):29. doi: 10.3390/antiox7020029

Figure 1.

Figure 1

Stability of ascorbate and DHA in PBS. (A) Ascorbate (50 µmol/L) was incubated on ice (■,▲) or at room temperature (☐,▽) in the absence (solid line) or presence (dashed line) of the metal chelator DTPA (100 µmol/L). (B) DHA (50 µmol/L) was incubated on ice (■,▲) or at room temperature (☐,▽) in the absence (solid line) or presence (dashed line) of DTPA (100 µmol/L). Samples were analysed for ascorbate spectrophotometrically (following reduction of DHA with DTT) at the indicated time points. Data represent the mean ± SEM (n = 3). Repeated measure one-way ANOVA with Dunnett’s test for multiple comparison showed no significant loss of ascorbate on ice over 6 h (p = 0.697), but a significant decrease at room temperature from 2 h (in the absence of DTPA, p = 0.030). A small but significant decrease in DHA on ice was observed from 3 h (p = 0.042) and a large decrease in DHA at room temperature was observed from 1 h (p = 0.068).