Table 4.
Comparative analysis of control plasmids’ characteristic, which may potentially contribute to antitumor effectiveness after in vivo GET.
| Plasmid | Base Pairs | Molecular Weight * | Copies/100 ug | Motifs **/Copy | Total Motifs ** Injected | Promotor | Antibiotic Resistance Gene |
|---|---|---|---|---|---|---|---|
| pVAX1 | 2999 | 1.85 × 106 | 3.25 × 1013 | 7 | 2.27 × 1014 | CMV | kanamycin |
| pControl | 3390 | 2.09 × 106 | 2.87 × 1013 | 10 | 2.87 × 1014 | CMV | ampicillin |
| pENTR/U6 scr | 2909 | 1.79 × 106 | 3.35 × 1013 | 13 | 4.36 × 1014 | U6 | kanamycin |
* with precise sequence, http://scienceprimer.com/nucleotide-molecular-weight-calculator; ** Calculation of number of mouse specific CpG motifs injected into tumors (100 ug DNA) per Yu et al., 2003; irrespective of relative immunostimulatory activity [40].