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. 2018 Feb 3;10(2):41. doi: 10.3390/cancers10020041

Figure 2.

Figure 2

RR cells had higher phosphorylation of Sox2. Immunoprecipitation experiments were carried out using RU and RR cells derived from MCF7 cells transfected with flag-SOX2. Precipitation was carried out using the anti-flag M2 affinity gel and Western blot was carried out using Sox2, p-Tyrosine and p-Threonine antibodies. (A) Right panel represents immunoprecipitation of flag-Sox2 pulled down in both RU and RR cells derived from MCF7 cells. Of note, two bands of Sox2 were identified in RR cells whereas only one band was identified in RU cells. Left panel represents Western blots results probed with anti-phospho-tyrosine (p-tyrosine) and anti-phospho-threonine (p-threonine) revealed evidence of Sox2 phosphorylation in RR but not RU cells; (B) Western blot of Sox2 was performed using 3 patient tumor samples. Immuno-reactivity with anti-p-tyrosine and anti-p-threonine was detected in all 3 samples.