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. 2018 Feb 3;10(2):42. doi: 10.3390/cancers10020042

Figure 3.

Figure 3

MYC regulates ITGA6 splicing through epithelial splicing regulatory protein 2 (ESRP2). Quantitative RT-PCR analysis of ESRP1 (a), ESRP2 (b) and heterogeneous nuclear RNA-binding protein H1 (hnRNPH1), hnRNPA1 and polypyrimidine tract-binding protein 1 (PTBP1) (c) mRNA expression in CRC T84 cells treated with the MYC inhibitor at 50 and 100 µM for 48 h. *: statistically significantly different (p ≤ 0.05) from DMSO control. Data are expressed normalized to RPLPO expression as a validated housekeeping gene. (d) Representative immunoblot analyses of ESPR2 protein level expression. β-actin (ACTB) was used as loading control. ESRP2 knockdown alters ITGA6 splice variant expression. (e) Representative immunoblot and densitometric analyses of ITGA6A, ITGA6B and ESRP2 in CRC cells knocked down for ESRP2. Cytokeratin 18 (K18) was used as loading control. **: statistically significantly different (p ≤ 0.01) from control sh (shCTL). 2.4. MYC Binds to ESRP2 and ITGA6 Promoters.