Skip to main content
. 2018 Feb 3;10(2):42. doi: 10.3390/cancers10020042

Figure 4.

Figure 4

MYC binds to the ITGA6 and ESRP2 promoters. Chromatin immunoprecipitation (ChIP) assays were performed on T84 lysates using either anti-human c-Myc-specific antibodies (a) or anti Pol-II antibodies (b). Mock anti-IgG were used as negative control. The cyclin D1 CCND1 promoter containing one complete E-box response element was used as a positive control. *: Statistically significantly different (p ≤ 0.05) from IgG. (c,d) Luciferase assay of the detection of ITGA6 promoter activity. The ITGA6 promoter coupled to Renilla luminescent reporter gene inserted into the pLightSwitch_Prom vector was transfected into HEK293T (c) or in CRC T84, Caco2-15 or SW680 (d) together with the empty vector (EV), a MYC expressing vector (MYC), the transcriptional dominant negative MADMYC (MAD) or both (MYC + MAD). Results showed the net Renilla/Firefly ratio relative to EV. * to ***: Statistically significantly different (p ≤ 0.05 to 0.001) from EV. (e) Quantitative RT-PCR analysis of MYC and ITGA6 expression in HIEC, T84, Caco2-15 and SW620 cells. * to ***: Statistically significantly different (p ≤ 0.05 to 0.001) from HIEC cells. (f) Quantitative RT-PCR analysis of ITGA6 expression in HIEC overexpressing MYC and representative immunoblot for MYC detection in the same cells. *: Statistically significantly different (p ≤ 0.05) from EV.