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. 2018 Feb 2;19(3):e45009. doi: 10.15252/embr.201745009

Figure 2. Markers of mitochondrial biogenesis, mitochondrial chaperones, and mitochondrial fission/fusion regulator OPA1 are elevated in gWAT of ClpP −/− mice.

Figure 2

  1. Left panel: immunoblots of gWAT extracts from WT, ClpP +/−, and ClpP −/− mice for PGC‐1α, Tfam, VDAC, β‐actin, and ClpP (n = 5). Right panel: graphical representation of quantified blots normalized to β‐actin.
  2. Quantification of protein levels of electron transport chain (ETC) subunits ATP5a1, ATP5ab, SDHA, SDHAB, SDHC, and UQCRC2 in gWAT of WT, ClpP +/−, and ClpP −/− mice obtained by mass spectrometry (n = 5).
  3. Quantification of mtDNA/nDNA content in gWAT from WT and ClpP −/− mice (n = 6–8).
  4. Top panels: immunoblots of sWAT, BAT, heart, and muscle extracts from WT and ClpP −/− mice for PGC‐1α and β‐tubulin (Western blot shows representative examples for n = 4, and quantification is based on n = 6). Bottom panels: graphical representation of quantified blots normalized to β‐tubulin.
  5. Left panel: immunoblots of gWAT extracts (30 μg/lane) from WT and ClpP −/− mice for 4‐HNE. Right panel: Quantification of band intensity of the entire line represented graphically (n = 5).
  6. Top panel: immunoblots of gWAT extracts from WT and ClpP −/− mice for P‐AMPK and AMPK (n = 5). Right panel: graphical representation of the ratio of P‐AMPK to AMPK.
  7. Left panel: immunoblots of gWAT extracts from WT, ClpP +/−, and ClpP −/− mice for Lon, Hsp60, Hsp40, Hsp10, ClpX, and ClpP (n = 5). Right panel: graphical representation of quantified blots normalized to β‐actin.
  8. Western blots showing protein expression of OPA1 isoforms and β‐actin in gWAT, sWAT, BAT, muscle and heart of WT, and ClpP −/− mice (top panel) (Western blot shows representative examples for n = 2, and quantification is based on n = 5). Quantification of the blots normalized to β‐actin is shown as bar graphs (bottom panel).
Data information: Bars represent mean ± SEM (ANOVA, *: WT vs. ClpP −/−; #: ClpP +/− vs. ClpP −/−; ^: WT vs. ClpP +/−; */#/^ P < 0.05). WT: white bars, ClpP +/−: gray bars, ClpP −/−: black bars.