Skip to main content
. 2018 Jan 18;19(3):e44799. doi: 10.15252/embr.201744799

Figure EV1. Impact of FBXL13 on cell cycle distribution and CEP192 expression levels.

Figure EV1

  1. Quantification of cell cycle profile of HEK293T cells as determined by fluorescence‐activated cell sorting (FACS) using propidium iodide staining. HEK293T cells were transfected with FBXL13 isoform 1 (FBXL13‐1) or FBXL13 isoform 3 (FBXL13‐3). An empty vector (Vector) was used as a negative control (mean ± SD from three independent experiments; ns, non‐significant P > 0.05). The statistical method used was a one‐sample, unpaired Student's t‐test with a hypothetical means of 1.0 between G2/M populations of the treatment conditions compared to control.
  2. Detection of CEP192, Centrin‐2, Centrin‐3 and CUL1 after expression of FBXL13 in the presence or absence of MG‐132. β‐actin was used as a loading control.