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. 2018 Jan 18;19(3):e44799. doi: 10.15252/embr.201744799

Figure EV3. FBXL13 regulates centrosome duplication and centrosomal microtubule nucleation.

Figure EV3

  1. Quantification of centrosome overduplication in U2OS cells transfected with a non‐targeting siRNA (siRNA NTC) or two siRNA oligos targeting FBXL13 (1 and 2) (mean ± SD from three independent experiments, n > 200 cells per condition; *P ≤ 0.05). Statistical analysis was performed by using one‐sample t‐test with hypothetical means of 1.0.
  2. Validation of FBXL13 mRNA level using quantitative real‐time PCR (qPCR) in the samples shown in (A) (mean ± SD from three qPCR triplicates).
  3. Microtubule regrowth assay in U2OS cells transfected with either Flag‐FBXL13 or an empty vector control. Microtubules were fully depolymerised by treating cells with 10 μM nocodazole for 2 h at 4°C. Cells were fixed 1 min after nocodazole washout and stained for Flag (red), α‐tubulin (green) and DNA (DAPI, blue). Scale bar, 10 μm.
  4. Quantification of cells which nucleated centrosomal microtubules after complete depolymerisation shown in (C) (mean ± SD from three independent experiments, n = 100 cells per condition and experiment, **P ≤ 0.01). Statistical analysis was performed by using one‐sample t‐test with hypothetical means of 1.0.