Figure 3. mTH⁺/HNK⁺ cells are immature CB neuroblasts.

• A–C
  Flow cytometric analysis of dispersed CB cells from normoxic (Nx), 5d hypoxic (Hx), or 21d hypoxic (Hx) rats, stained for TH and HNK‐1. Note how mTH⁺/HNK⁺ cells convert into TH⁺/HNK⁻ mature glomus cells upon exposure to hypoxia.
• D
  Quantification of the flow cytometry analysis shown in (A–C) (n = 5–7 rats per group).
• E
  Flow cytometry analysis of Tuj1 expression in dispersed CB cells from normoxic animals. Note how mTH⁺/HNK⁺ cells express higher levels of Tuj1 than mature TH⁺/HNK⁻ cells, confirming their immature phenotype.
• F
  Quantification of the mean Tuj1 expression in TH⁺/HNK⁻ mature glomus cells versus mTH⁺/HNK⁺ immature neuroblasts (n = 3 independent experiments with a total of 10 rats).

Data information: Data in bar graphs are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 (Student's t‐test). Source data are available online for this figure.