Figure EV5. Hypoxia and cholinergic signaling induce neuroblast maturation.

• A–D
  Increase in NAD(P)H autofluorescence in response to hypoxia in mature glomus cells (TH) after 24–48 h in normoxia (A), in immature neuroblasts (mTH/HNK) after 24–48 h in normoxia (B), in immature neuroblasts after 24–48 h in hypoxia (C), and in immature neuroblasts after exposure to acetylcholine (ACh) for 24–48 h (D).
• E
  Quantification of hypoxia‐responsive cells, as measured by NAD(P)H autofluorescence, under the indicated conditions. Number of total cells studied is indicated between brackets.
• F
  Quantification of the magnitude of the response to hypoxia in the different cells under the indicated conditions. Number of total cells studied is indicated between brackets.

Data information: Data in bar graph of panel (E) are presented as the sum of responding cells among total cells studied. Data in bar graph of panel (F) are presented as mean ± SEM. *P < 0.05, **P < 0.01 (Student's t‐test). Cells used for the analysis in this figure were obtained in eight independent experiments with four rats each.

Source data are available online for this figure.