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. 2018 Jan 12;19(3):e44867. doi: 10.15252/embr.201744867

Figure 1. Isolation of hypermating and temperature‐sensitive growth mutants.

Figure 1

  1. The affected genes of hmt mutants.
  2. Mating efficiency of hmt mutants on nutrient‐rich medium. Cells of wild‐type (WT, JY450), nrs1/hmt1 (JS159), prs1/hmt2 (JS160), tad3/hmt3 (JS161), rpc34/hmt4 (JS162), sfc4/hmt5 (JS163), cts1/hmt6 (JS164), pcm1/hmt7 (JS165), and tor2‐ts6 (JV303) strains were grown on YE medium at 25 or 30°C for 5 days, and mating frequency was measured. Mean ± SD values of three independent measurements are shown (total n > 300).
  3. TORC1 activity in hmt mutants. Cells of wild‐type, tor2‐ts6, nrs1/hmt1, prs1/hmt2, tad3/hmt3, rpc34/hmt4, sfc4/hmt5, cts1/hmt6, and pcm1/hmt7 strains were grown in liquid YE medium at 25°C and then shifted to 34°C for 4 h. Cell extracts were subjected to Western blot analysis using anti‐Atg13 antibody and anti‐phospho‐S6 kinase antibody. γ‐tubulin is shown as a loading control.