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. 2018 Jan 12;19(3):e44867. doi: 10.15252/embr.201744867

Figure EV2. The extent of aminoacylation and amount of total tRNA do not change under nitrogen starvation.

Figure EV2

  1. Aminoacylation of tRNA‐Asn under nitrogen starvation. Wild‐type (JY3) cells were cultured in minimal medium, MM, and shifted to nitrogen‐deprived MM for 30 min. RNA extracted in acidic conditions was analyzed by northern blot analysis with probes for tRNA‐Asn. The rightmost lane is deacylated wild‐type RNA (DA). 5S rRNA is shown as a loading control.
  2. Amount of total tRNA under nitrogen starvation. Total RNA was separated, followed by ethidium bromide staining.