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A
Sla1 overexpression in tor2‐ts6 mutant cells. tor2‐ts6 cells (JV303) carrying pREP1 or pREP1‐Sla1 were incubated on MM medium at 25°C for 4 days.
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B
Mating efficiency of cells overexpressing constitutive active TORC1 mutant (Tor2‐s69). tor2‐ts6 (JV303), nrs1/hmt1 (JS159), prs1/hmt2 (JS160), tad3/hmt3 (JS161), rpc34/hmt4 (JS162), sfc4/hmt5 (JS163), cts1/hmt6 (JS164), pcm1/hmt7 (JS165), ths1 (JS167), and lrs1 (JS168) cells carrying pREP1 or pREP1‐tor2‐s69 were incubated on MM medium at 30°C for 2 days, and mating frequency was measured. Mean ± SD values of three independent measurements are shown (total n > 300).
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C–F
Mating efficiency of cells overexpressing pre‐tRNA in prs1, ths1, rpc34, and pcm1 mutant cells. prs1, ths1, rpc34, and pcm1 cells carrying pREP1 or pREP1‐pre‐tRNA‐Leu‐intr‐RZ were incubated on MM medium at 30°C for 2 days (C and D) or 3 days (E and F), and mating frequency was measured. Mean ± SD values of three independent measurements are shown (total n > 300). *P < 0.05; **P < 0.01 (Student's t‐test).
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G
Mating efficiency of cells overexpressing Gtr1QL in nrs1, prs1, rpc34, ths1, and lrs1 mutant cells. Wild‐type (WT), nrs1, prs1, rpc34, ths1, and lrs1 cells carrying pREP1 or pREP1‐Gtr1Q61L were incubated on MM medium at 30°C for 2 days, and mating frequency was measured. Mean ± SD values of three independent measurements are shown (total n > 300).
