Figure 2.

Effects of Pck1 on LPS-induced glycolytic pathway. BMDMs isolated and differentiated from Pck1^flox/flox and Pck1^MC-KO mice were incubated with LPS for 4 h. A, 2-[U-¹⁴C]deoxyglucose uptake was measured and normalized by protein concentration. Values represent the mean ± S.E. for n = 6 per group. *, p < 0.05 compared with vehicle group with the same genotype. B, mRNA expression of glycolytic enzymes was measured by RT-PCR. The values are the mean ± S.E. for n = 6–8 per group normalized with 18S rRNA and expressed as -fold difference over Pck1^flox/flox. C, lactate concentrations from cell lysates were determined by GC-MS. Values represent the mean ± S.E. for n = 6 per group. *, p < 0.05 compared with Pck1^flox/flox group. GLUT1, glucose transporter 1; PFK2, phosphofructokinase 2; LDH, lactate dehydrogenase.