Figure 5.

De novo lipogenesis in LPS-activated macrophages. A–E, BMDMs isolated and differentiated from Pck1^flox/flox and Pck1^MC-KO mice were incubated with [²H₂O] (A and B) or [3-¹³C]lactate (D and E) in the presence of LPS for 16 h. GC-MS was performed for lipid-bound glycerol and palmitate. The values are expressed as newly synthesized or abundance (MPE%) as indicated. Values represent the mean ± S.E. for n = 3 per group. C, BMDMs isolated and differentiated from Pck1^flox/flox and Pck1^MC-KO mice were incubated with LPS for 4 h. mRNA expression of lipid metabolism genes were measured by RT-PCR. F, BMDMs isolated from Pck1^flox/flox and Pck1^MC-KO mice were incubated with LPS for 4 h and total triglyceride concentration in the cells was measured. The values are the mean ± S.E. for n = 6–8 per group normalized with 18S rRNA and expressed as -fold difference over Pck1^flox/flox. *, p < 0.05. FASN, fatty acid synthase; ACC, acetyl-CoA carboxylase; CD36, cluster of differentiation 36.