Figure 4.
NMR analysis of LTA isolated from B. subtilis wildtype 168, yfhO mutant, and complementation strains. A–C, NMR spectra of LTA obtained from B. subtilis 168 (WT) (A), the yfhO mutant (B), and the yfhO+csbBD97A-yfhO complementation strain (C). Colored boxes and labels indicate nonexchangeable protons derived from the different LTA components. Peaks were assigned as described previously (17, 37–39). The different peaks for the protons and acetyl group of GlcNAc are labeled with 1H, 4H, and Ac, respectively. Gray boxes highlight peaks resulting from residual citrate, a buffer component used during the LTA purification procedure. The spectra are representative of three independent experiments. The spectrum for the B. subtilis 168 (WT) LTA is the same as shown in Fig. 2A.