Figure 4.

Endosomal sorting genes are key negative regulators of ROMK at the cell surface. A, representation of the synthetic genetic array screen. B, GO term analysis of very strong hits (z-score >3) isolated from initial screen listed in increasing order of p values from the most significant (<0.001) to the least significant (<0.05). C, endosomal transport genes considered for further investigation in this study. Genes are arranged by functional classification. Genes in boldface were identified in the screen, whereas those in normal type were selected as known interactors with identified genes. Underlined genes were selected for de novo strain reconstruction.