Figure 5.

Deletion of genes encoding select ESCRT and CORVET components improves the growth of potassium-sensitive yeast expressing ROMK1_S44D+K80M. Yeast strains with the indicated genotypes transformed with an empty expression vector control or vectors engineered to express Kir2.1, wildtype ROMK1, or ROMK1_S44D+K80M were serially diluted onto medium supplemented with the indicated amounts of KCl. All strains in this figure were identified in the high-throughput SGA screen and then reconstructed de novo. All experiments were performed in duplicate, and representative serial dilutions are shown.