Figure 7.

ARRDC3 re-expression attenuates PAR1-stimulated JNK signaling. MDA-MB-231 HA-ARRDC3 pSLIK cells treated with or without 1 μg/ml DOX for 48 h were stimulated with 10 nm α-thrombin for the indicated times. Cells were lysed and immunoblotted for phospho-JNK1/2, total JNK, HA-ARRDC3, and β-actin expression. The data shown (mean ± S.D. (error bars), n = 3) were quantified by densitometry and are represented as the -fold increase in JNK1/2 phosphorylation relative to 0 min control. Statistical significance was determined by one-way ANOVA (**, p < 0.01; ****, p < 0.0001; n = 3).