Figure 1.

LplT/Aas-mediated LPL acylation in E. coli. a, cartoon model showing lysophospholipid transporter LplT importing LPE from the periplasm across the cytoplasmic membrane for acylation by acyltransferase Aas to generate PE. b, LPE acylation assay of E. coli W3110 WT, ΔlplT, and Δaas strains. The activities were calculated based on conversion (%) of [³²P]LPE to [³²P]PE shown on TLC images in Fig. S2. c, LPE transport assays using spheroplasts generated from E. coli W3110 WT, ΔlplT, and Δaas cells. The transport activity of LplT in each sample was calculated based on the acquired radioactivity in the spheroplasts.