Figure 2.

Deletion of lplT or aas gene increases susceptibility of E. coli to psPLA₂. a, viability tests of E. coli W3110 WT, ΔlplT, and Δaas strains treated with 12 units of psPLA₂, 20 μl of AF, or 20 μl of cationic protein-depleted AF for 90 min. b, time-course viability assays of E. coli W3110 WT, ΔlplT, Δaas, and Δaas/lplT strains (with 12 units psPLA₂, with 20 μl of AF). c and d, titration experiment of E. coli viability in the presence of 12 units of psPLA₂ and indicated volume of AF (c) and in the presence of 20 μl of AF and psPLA₂ (d) as indicated. The reaction time was 90 min. All data are expressed as CFUs (%) compared with untreated WT control at zero time.