Figure 3.

E. coli resistance is determined by the function of LplT and Aas. a, LPE acylation assay of E. coli W3110, lplT^D30A, and aas^H36A strains. The activities were calculated based on conversion (%) of LPE to PE shown on TLC images in Fig. S2. b, LPE transport assays using spheroplasts generated from E. coli W3110 WT, lplT^D30A, and aas^H36A cells. The transport activity of LplT in each sample was calculated based on the acquired radioactivity in the spheroplasts. c, viability tests of E. coli W3110 WT, lplT^D30A, and aas^H36A strains (+ 12 units of psPLA₂, ± 20 μl AF) for 90 min. The data are expressed as CFU (%) compared with untreated WT control. d, Western blotting analysis using anti-His antibody to examine LplT^WT, LplT^D30A, Aas^WT, or Aas^H36A protein expressed in E. coli. Cell lysates containing the same amount of total protein were loaded for each sample.