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Alterations of the outer membrane in ΔlplT and Δaas strains. a, chemical structure of amphiphilic fluorescent probe NR12S. b, emission spectra of fluorescent probe NR12S in W3110 WT, ΔlplT, and Δaas cells after excitation at 530 nm. c, assay of bacterial OM asymmetry by determining PE at the bacterial surface. d, changes in fluorescent intensity following the addition of the Ro 09-0198–PEG5000–Alexa 488 conjugate into W3110 WT and mutant cells are shown.
