Skip to main content
. 2018 Jan 18;293(9):3335–3349. doi: 10.1074/jbc.M117.816280

Figure 2.

Figure 2.

SEC-MALS and CD spectroscopy analysis of different VP40 proteins employed in in vitro lipid-binding measurements. SEC-MALS analysis was used to confirm the stoichiometry of VP40 and mutants in solution. A, table of WT VP40 and mutants confirming the molecular weight of each species as calculated within the SEC-MALS trace. Asterisk indicates that a small secondary population of higher-order structure was observed in these samples. B, SEC-MALS trace of FL WT VP40. C, SEC-MALS trace of ΔGEEGΔ. D, CD spectroscopy analysis of WT VP40 and mutants confirming the agreement of α-helical and β-sheet content among all constructs.