Figure 3.

A, effects of the STIM1 L390Q mutation on AA-activated ARC channel currents either alone or when included with T389E mutation. B, effects of the STIM1 L390Q mutation on adenophostin-activated CRAC channel activity either alone or when included with T389L mutation. Whole-cell inward ARC and CRAC channel currents (error bars represent the mean ± S.D.; n = 4–6) were measured at −80 mV using the standard protocols in WT cells and in the same cells bearing the STIM1 L390Q mutation and the same mutation along with either the T389E mutation (ARC channels; A) or the T389L mutation (CRAC channels; B). Individual measurements are indicated by open circles. ARC channel and CRAC channel currents were activated by standard procedures (see “Experimental procedures”).