Figure 1.

Characterization of the 1660 [URE3] strain. A, the 1660[URE3] and the cured 1660[ure-o] yeast strains were imaged and plated. The 1660[URE3] strain was cured in 5 mm guanidine to make the 1660 [ure-o]. These strains were imaged on the Zeiss 880 microscope and plated on ½ YPD plates. Images are maximized projections of Z-stack confocal images. B, images of 1660 [URE3] strain during curing in guanidine. Confocal images were obtained using the Zeiss 880 microscope after incubation in guanidine for two, four, or eight generations (gen), respectively. The images are the maximum Z-stack projections. The same settings were used in all images. C, the kinetics of curing the 1660[URE3] and 1076[URE3] yeast strains in 5 mm guanidine was measured using the red/white colony assay. The cells at the indicated time were plated on ½ YPD plates, and only completely red colonies were counted as cured. The data represent the averages and standard deviation obtained from three independent experiments. The cells with foci were measured in 1660 [URE3] yeast following the addition of 5 mm guanidine (>300 cells were counted/time point). The percentage of cells with foci as a function of generation time are plotted on the same graph as the curing curves.