Figure 5.

Inhibition of mCOX-2 with ARN2508 with or without preincubation. Oxygenation of 5 μm AA (A) or 5 μm 2-AG (B) by mCOX-2 was assessed by quantification of enzymatic product formation utilizing LC-MS/MS as described under “Experimental procedures.” Each enantiomer of ARN2508 was preincubated for 10 min (10 m) or added simultaneously (0 m) with substrate. Each substrate was allowed to react for 10 s before quenching with organic solvent containing deuterated internal standards. Results are the mean ± S.D. of triplicate determinations. Some error bars are shorter than the height of the symbol.