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. 2018 Jan 3;293(9):3281–3292. doi: 10.1074/jbc.RA117.000298

Figure 4.

Figure 4.

Relative contribution of EPHX1 and EPHX2 to hydrolysis during EET formation in liver microsomes. Microsomes made from livers of WT, Ephx1−/−, Ephx2−/−, and Ephx1−/−Ephx2−/− mice were incubated with 10 μm arachidonic acid in the presence/absence of increasing concentration of NADPH. Relative formation of 11,12-DHET (A), 11,12-EET (B), 14,15-DHET (C), 14,15-EET (D), 8,9-DHET (E), and 8,9-EET (F) at 0–200 μm NADPH are shown as a percentage of WT (A, C, E) or Ephx1−/−Ephx2−/− (B, D, F). Formation rates at each NADPH concentration are shown above each group in pg/mg protein/min. n = 4 per group, *, p < 0.05 versus WT; #, p < 0.05 versus Ephx2−/−. Results are expressed as mean ± S.E.