Figure 2.
Rx1 binds NbGlk1 in vitro. A, interaction of Rx1(1–144) with NbGlk1. On the left are representative gel-filtration chromatograms of Rx1, NbGlk1(83–402), and Rx1 incubated with NbGlk1(83–402). Peak fractions were visualized by SDS-PAGE. B, interaction of Rx1(GST-1–144) with NbGlk1. On the left are representative gel-filtration chromatograms of Rx1(GST-1–144), NbGlk1(83–402), GST, and Rx1(GST-1–144) incubated with NbGlk1(83–402) and GST incubated with NbGlk1(83–402). Peak fractions were visualized by SDS-PAGE.