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. 2017 Dec 7;293(9):3218–3233. doi: 10.1074/jbc.RA117.000485

Figure 3.

Figure 3.

Rx1 binds NbGlk1 in planta. Shown is co-immunoprecipitation of 4×Myc-tagged full-length Rx1 or Rx1–CC when co-expressed in planta with C-terminally HA-tagged NbGlk1. The labels on the figure are as follows: Input denotes the constructs agroinfiltrated into N. benthamiana leaves; αMyc denotes an immunoblot probed using an anti-Myc epitope tag antibody; αHA denotes an immunoblot probed using an anti-HA epitope tag antibody; αMyc IP denotes immunoprecipitation of the denoted input samples using an anti-Myc epitope tag antibody; CBB denotes Coomassie Blue stain loading control for the denoted input samples. Immunoblot bands corresponding to Rx1-4myc, Rx1–CC-4myc, and NbGlk1-HA from the αMyc and αHA immunoblots are indicated.