Figure 7.

Binding of Rx1 and NbGlk1 protein to DNA in situ. A, representative confocal images of nuclei and surrounding cytoplasm of N. benthamiana epidermal cells transiently co-expressing NbGlk1-GFP with mCherry. Images are of the NbGlk1-GFP channel (left-hand panel), the mCherry channel (center panel), and an overlay of the NbGlk1-GFP and mCherry channels (right-hand panel). Co-expression was performed in the presence (upper panels) or absence (lower panels) of the p19 silencing suppressor. Scale bar represents a width of 10 μm. Subcellular structures are indicated by N = nucleus; n = nucleolus; C = cytoplasm; V = vacuole; Ch = choloroplasts. B, ratio of the long to short GFP lifetimes for a Rx1-GFP full-length construct alone and upon co-expression with NbGlk1 and the avirulent CP106 allele of the PVX CP (scatter plot ± S.D.; bars with different letters are significantly different (p < 0.05); one-way ANOVA with post hoc Dunnett's multiple comparison). C, ratio of the long to short GFP lifetimes for NbGlk1-GFP full-length construct alone and upon co-expression with Rx1 and the avirulent CP106 allele of the PVX CP (scatter plot ± S.D.; bars with different letters are significantly different (p < 0.05); one-way ANOVA with post hoc Dunnett's multiple comparison).