Figure 8.

Influence of Rx1 interactors on the immune response to PVX. A, average cell death score for N. benthamiana leaves expressing NbGlk1 expressed in combination with CP106 and Rx1. Leaves were agroinfiltrated with A. tumefaciens transformed with constructs pBIN35S-CP106, pBIN35S-Rx1, and pBIN35S-NbGlk1-4HA. The pBIN35S-CP106 construct expresses only the PVX CP106 coat protein (scatter plot ± S.D.; letters indicate statistically different data sets (p < 0.05) calculated using Dunnett's multiple comparisons test). B, representative N. benthamiana leaf of the results provided in A. C, representative images of N. benthamiana leaf areas for each cell death score. The number within each image indicates the assigned score for the associated degree of cell death. D, ratio of fluorescence compared with a control of GFP expressed from a PVX amplicon for N. benthamiana leaves expressing GFP from a PVX amplicon in combination with NbGlk1 and Rx1. Leaves were infiltrated with A. tumefaciens transformed with pGR106 with and without constructs pBIN35S-Rx1 and pBIN35S-NbGlk1-4HA. pGR106 encodes a PVX amplicon in which GFP expression is driven from a duplicated coat protein promoter (scatter plot ±S.D.; bars with different letters are significantly different (p < 0.05); one-way ANOVA with post hoc Dunnett's multiple comparison).