Table 1.
Influence of hexahistidine-tagged Rx1 proteins on the dissociation constant (Kd) of NbGlk1 for DNA-binding motifs
Values are in μm (±S.D.) ND = not determined; – = no additional Rx1 protein; *, p < 0.0001 compared with the No site motif; #, p > 0.05 compared with the No site motif (F test).
|
NbGlk1(1–243) |
NbGlk1(83–243) |
NbGlk1(83–402) |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| – | Rx1(1–144) | Rx1(1–489) | – | Rx1(1–144) | Rx1(1–489) | – | Rx1(1–144) | Rx1(1–489) | |
| No site | ND | ND | ND | 0.33 ± 0.02 | 0.85 ± 0.27 | 1.34 ± 0.42 | 0.18 ± 0.01 | 0.40 ± 0.02 | 0.42 ± 0.09 |
| AGATTT | ND | ND | ND | 0.23 ± 0.01# | 0.76 ± 0.52 | 0.65 ± 0.04 | 0.16 ± 0.01# | 0.46 ± 0.06 | 0.34 ± 0.04 |
| GATATC | >1 | >1 | >1 | 0.18 ± 0.01# | 0.40 ± 0.15 | 0.50 ± 0.03 | 0.08 ± 0.00* | 0.30 ± 0.07 | 0.19 ± 0.04 |