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Influence of GST-tagged Rx1 protein on the dissociation constant (Kd) of NbGlk1(83–402) for DNA-binding motifs
Values are in μm (±S.D.); – = no additional Rx1 protein; *, p < 0.0001 compared with the No site motif (F test).
|
NbGlk1(83–402) |
||
|---|---|---|
| – | Rx1(GST-1–144) | |
| No site | 0.32 ± 0.03 | 1.99 ± 0.09 |
| AGATTT | 0.22 ± 0.00* | 0.73 ± 0.07 |
| GATATC | 0.11 ± 0.00* | 0.34 ± 0.00 |
