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. Author manuscript; available in PMC: 2018 Mar 5.
Published in final edited form as: J Investig Med. 2017 Nov 3;66(1):1–6. doi: 10.1136/jim-2017-000622

Figure 2.

Figure 2

Ubiquitin-specific protease 50 (USP50) is increased during erythropoiesis. (A) Total RNA was extracted from human CD34+ hematopoietic progenitor cells (HPCs) on day 1 and day 3 of erythroid differentiation. Real-time quantitative PCR was used to compare ubiquitin-specific protease mRNA levels. Amplification of each sample was normalized to its glyceraldehyde-3-phosphate dehydrogenase (GAPDH) transcript levels. Shown are the ratios of day 3/day 1 in a representative experiment from five independent experiments. (B) Total RNAs were extracted on days 1, 3, 5, and 9 from HPCs. USP50 mRNA levels were measured by real-time qPCR. Shown are data in a representative experiment from five independent experiments. (C) Whole cell lysates were made from HPCs on days 0, 1, 3, 5, and 9 of erythroid differentiation. USP50 and β-actin proteins were detected by immunoblot. Representative blots were shown from five independent experiments.